Skip to main content
SpringerLink
Log in

Liquid chromatography of gramicidin

  • Originals
  • Column Liquid Chromatography
  • Published:
Chromatographia Aims and scope Submit manuscript

Summary

A simple, selective method is described for separation of more than 10 gramicidin components on Spherisorb ODS B, 5 μm,250×4.6 mm I.D. column, maintained at 50°C. The mobile phase comprised methanol-water (71:29) at a flow rate of 1.0 mL min−1. Detection was by UV at 282 nm. Valine gramicidins A, B and C were very well separated from the isoleucine gramicidins A, B and C. Four new gramicidin components were also resolved and their structures determined by liquid chromatography/ mass spectrometry. The names 10-methionine valine gramicidin C, 4-methionine valine gramicidin A, valine gramicidin A hydroxypropyl and isoleucine gramicidin A hydroxypropyl were proposed. Robustness of the liquid chromatography method was evaluated by performing a full factorial design experiment. The method also showed good repeatability, linearity and sensitivity.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Brewer, G.A. in: Florey, K. (Editor),Anal. Profiles of Drug Substances, Vol. 8, Academic Press, New York,1979, p. 179.

    Google Scholar 

  2. Martin, A.R.Antibacterial Antibiotics, in: Delgado, J.N.; Remers, W.A. (Editors), Wilson and Gisvold's Textbook of Organic Medicinal and Pharmaceutical Chemistry, 10th edition, Lippincott-Raven, Philadelphia,1998, p. 253.

    Google Scholar 

  3. Drucker, D.B. (Editor),Microbiological application of high-performance liquid chromatography, Cambridge University Press, London,1987, p. 123.

    Google Scholar 

  4. Gregory, J.D.; Craig, L.C.,J. Biol. Chem. 1948,172, 839.

    CAS  Google Scholar 

  5. Okamoto, K.; Yonezawa, H.; Izumiya, N.J. Chromatogr. 1974,92, 147.

    Article  CAS  Google Scholar 

  6. Koeppe II, R.E.; Weiss, L.B.J. Chromatogr. 1981,208, 414.

    Article  CAS  Google Scholar 

  7. Govaerts, C.; Orwa, J.A.; Van Schepdael, A.; Roets, E.; Hoogmartens, J.Rapid Commun. Mass Spectrom., submitted.

  8. Axelsen, K.S.; Vogelsang, S.H.J. Chromatogr. 1977,140, 174.

    Article  CAS  Google Scholar 

  9. European Pharmacopoeia, 3rd edition, Council of Europe, Strasbourg, France,1997.

  10. United States Pharmacopeia 24, United States Pharmacopeial Convention, Rockville, MD,1999.

  11. Molnar, I.; Horvath, C.J. Chromatogr. 1977,142, 623.

    Article  CAS  Google Scholar 

  12. Fisher, B.V.; Raja, R.B.Anal. Proc. 1982,19, 137.

    CAS  Google Scholar 

  13. Adams, E.; Schepers, R.; Gathu, L.W.; Kibaya, R.; Roets, E.; Hoogmartens, J.J. Pharm. Biomed. Anal. 1997,15, 505.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Faculteit Farmaceutische Wetenschappen, Laboratorium voor Farmaceutische Chemie en Analyse van Geneesmiddelen, Kátholieke Universiteit Leuven, Van Evenstraat 4, 3000, Leuven, Belgium

    J. A. Orwa, C. Govaerts, E. Roets, A. Van Schepdael & J. Hoogmartens

Authors
  1. J. A. Orwa
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. C. Govaerts
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. E. Roets
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. A. Van Schepdael
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. J. Hoogmartens
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Orwa, J.A., Govaerts, C., Roets, E. et al. Liquid chromatography of gramicidin. Chromatographia 53, 17–21 (2001). https://doi.org/10.1007/BF02492421

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02492421

Key Words

Search

Navigation